HIV-1 p24 ELISA Kit, 96 wells
HIV-1 p24 ELISA Kit, 96 Wells – High-Sensitivity Detection for Viral Quantification The HIV-1 p24 ELISA Kit (96 wells) is a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) designed for the quantification of HIV-1 p24 antigen in cell culture supernatants and lentiviral samples. This assay enables accurate monitoring of HIV-1 viral load, making it a valuable tool for virology and gene therapy research.
Assay Principle:
This ELISA utilizes murine anti-HIV-1 p24 capture antibodies pre-coated onto microtitration wells. When test samples containing HIV-1 p24 antigens are introduced, they bind to the immobilized antibodies. After an incubation period, unbound components are washed away.
A mouse anti-HIV-1 p24 antibody conjugated with biotin is then added, allowing further specificity in antigen detection. Following a second wash, a streptavidin-HRP conjugate is introduced to bind the biotinylated antibodies. After a third wash step, the addition of tetramethylbenzidine (TMB) substrate generates a colorimetric reaction proportional to the concentration of p24 antigen. The assay results are measured spectrophotometrically, providing quantitative data for viral load analysis.
Applications:
✔ HIV-1 Viral Load Monitoring in research studies
✔ Lentiviral Titer Determination in gene therapy and vaccine development
✔ Preclinical and Laboratory Research
Kit Presentation
Materials Supplied :The reagents supplied in this pack are for research use only.
1-Coated microwell strips - 1 plate
Plastic microtitration wells coated with antiHIV-1 p24 murine monoclonal antibody in foil pouch with desiccant - 96 wells
2-Positive HIV-1 p24 Control (10ng/ml) - 0.1 ml
3-Lysis Buffer - 6 ml
4 Detector Antibody. Anti-HIV-1 p24 conjugated to biotin - 12 ml
5 Conjugate. Streptavidin conjugated to horseradish peroxidase enzyme containing 0.01% Bromonitrodioxane as preservative - 12 ml
6-Wash Buffer (20X concentrated). Tris buffered saline pH 7.8-8.0, containing 0.05% Tween 20. Must be diluted before use. 2 bottles - 60 ml
7-Substrate solution. Tetramethylbenzidine - 12 ml
8-Stop Solution. 1 N H2SO4 - 12 ml
Storage and Stability
All reagents should be stored at 2-8 °C and should not be used beyond the expiration date on the label. Once opened, microtitration strips may be stored at 2-8 °C until the expiration date on the label, provided that desiccated conditions are maintained. Unused strips should be returned to their original foil pouch along with the sachet of desiccant. Secure open foil pouch using zip top before storage. The working strength Wash Buffer should not be stored for longer than 3 weeks at 2-8 °C . It is recommended that Wash Buffer be freshly diluted before each assay. If the working strength buffer becomes visibly cloudy or develops precipitate during the 3 weeks, do not use it.